الفهرس 
REVIEW OF LITERATUREOnly 14 pages are availabe for public view
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Abstract
The study of this thesis included the method of Nerium oleander propagation and how to improve the production of steroidal glycosides (oleandrin) as a secondary product of this plant which is considered as the principal drug available for treatment of cardiovascular disorder. The objectives of this study were to investigate the effect of explant, growth regulators, medium contents, carbon source and precursor on Nerium oleander callus production and its glycosidal content using tissue culture technique. The research was carried out in the tissue culture laboratory of the Horticulture Department, Faculty of Agriculture, Mansoura University during the season of 19992004. The part of analysis and determination of oleandrin was carried out in Faculty of Pharmacy, Cairo University. This study revealed the following important results: 1The best method for sterilization was by 20% commercial clorox (15 min). 2Increased multiplication was through using 3/4 MS and 0.5 mg/L BAP. 3The largest callus was by treating with 2 mg/L 2,4D and 2 mg/L kinetin. 4The highest amount of oleandrin was achieved from callus derived from leaf explant grown on MS media with 30 g/L sucrose o.594 mg (0.3%). 5The percentage of oleandrin which was considered the most important objective in this work was performed by treating leaves and nodes with (0.5 mg/L biotin + 6 mg/L 2,4D + 0.5 mg/L folic acid). Accordingly, increasing callus dry weight induced the largest amount of this active constituent. 6 The highest percentage (0.8%) and quantity of oleandrin was (0.960 mg) in callus nodes cultured on MS medium supplemented with 1o6 <U+00B5>M/l. GA3 and 3 mg/L 2,4D. 7The highest percentage of 0.8% which was achieved from the medium supported with the high concentration of progesterone (1 mg/L).