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Abstract Nosocomial infections are those which developed 72 hours after admission to hospital in a patient who was symptom free at time of admission. It is serious, world wide problem because it increases treatment costs, prolongs hospital stay and leads to spread of infections. RSV is the most common cause of nosocomial bronchiolitis and pneumonia especially in children and young infants. Aim of the work was to evaluate the incidence of nosocomial RSV in the Neonate intensive care unit in Mansoura University Hospital. Samples were Broncho-alveolar aspirate (BAA) and were subjected for detection ofRSV antigens by: 1- Direct .immunoflourescence technique. 2- Immunofluorescence 48 hours after cell culture. Out of 295 patients admitted to NICU in MUH from January to . May 1997., 47 patients (15.93%) developed nosocomial respiratory distress. By using DIF technique, out of 47 patients suffering from RD, 33 patients (70.2%) were positive for RSV, 13 cases (27.7%) were weak |