الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
P. aeruginosa is one of the most frequently isolated nosocomial pathogens. It has high interinsic resistance to many antibiotics. Because of high prevalence of P. aeruginosa in the hospital environment ,epidemiological investigations are facilitated by the use of markers that discriminate among strains. Genotypic methods have adequate discriminatory capacity in differentiation between different strains. The aim of this study was to estimate the infection rate of P. aeruginosa as a causative agent of nosocomial infection in M.U.H. and to investigate the relation ship between clinical isolates using genotypic methods. Total of 1196 samples were taken from patients fulfilling the criteria for defining nosocomial infection. These samples were transported as early as possible to the laboratory where the samples were cultured on different bacteriological media according to the type of the sample. Identification was done by colonial morphology, Gram-stained smears jpigment production, oxidase test and gelatin liquefaction test. Antibiotic susceptibility testing was done by using disc diffusion method as based on the original Kirby-Baeur method .Restriction endonuclease analysis was done using EcoRI enzyme. In this study, out of 1196 different clinical specimens collected from patients at different Clinical Wards, 181(15.1%) P. aeruginosa isolates were recovered. Burn and wound exudates were the commonest type of samples from which P. aeruginosa was recovered (40%), followed by ear