الفهرس 
b-lactam antibiotics and b-lactamasesOnly 14 pages are availabe for public view
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Abstract
Introduction: Metallo-B-lactamases are produced by bacteria as extracellular or periplasmic enzymes. These enzymes render the corresponding strains resistant to ?-lactam antibiotics. The rates of occurrence and the types of MBL clearly have escalated since 2000, severely limiting treatment options in Asia, Europe and Latin America. The fact that they hydrolyze almost all -lactam antibiotics, including carbapenems, underlines their clinical relevance. This work was planned for Identification of Gram-negative bacilli causing nosocomial infections in ICUs of Mansoura Emergency Hospital, qualitative detection of MBL production by imipenem-EDTA double disc synergy test and Hodge test and genetic detection of the MBL (blaIMP) gene by polymerase chain reaction. Conclusions: ” Gram-negative bacilli are the main causative agents of nosocomial infections in ICUs. ” The most common type of infection caused by Gram-negative bacilli in ICUs is LRTI. ” Metallo- -lactamases are one of the main causes of carbapenems resistance in ICUs. ” Imipenem EDTA double disc synergy test and Hodge test are easy, simple and specific tests for detection of MBL production. ” Pseudomonas species are the main MBL producing Gram-negative bacilli in ICUs. ” Long duration of ICU stay, mechanical ventilation and previous use of imipenem, meropenem and third generation cephalosporins are significant risk factors for acquisition of MBL producing isolates. ” Restricting the use of carbapenems, along with implementation of infection control measures are the most effective means of controlling the spread of MBL producing isolates in ICUs.