الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 117 |  |  |
| | | from | 117 | | |
Abstract
Three experimentswere carried out. The first experiment was planned to study the effects of different extenders (glucoseyolkcitrate: GYC, lactoseyolkcitrate: LYC, trisyolkfructose: TYF and skimmilkcitrate: SMC) on bull semen quality and fructose content, during chilled storage at 50C. The second experiment was carried out to study the effects of removal of seminal plasma by centrifugation and addition of different concentrations of caffeine (0, 2, 4 and 8mM/100ml) to the extended buffalo bull semen with LYC extender on semen quality, enzymatic activities and conception rate during chilled storage at 50C. The third experiment was carried out to establish the optimum condition of osmotic pressure using Nacitratelactose solution at different osmolarities (50, 100, 150, 200 and 300mOsmol/L) with either centrifuged or noncentrifuged buffalo semen. The results showed that, LYC and TYF extenders significantly (P<0.01) improved buffalo bull semen quality and significantly (P<0.01) decreased fructose content as compared to GYC or SMC extenders, during storage at 50C (Experiment, 1). Removal of seminal plasma by centrifugation of buffalo bull semen significantly (P<0.01) better semen quality and significantly (P < 0.01) decreased the leakage of GOT, GPT and ALP enzymes into the extracellular medium as compared to the noncentrifuged semen. Supplementation of 2, mM caffeine to the extended buffalo bull semen either centrifuged or noncentrifuged with LYC extender significantly (P<0.01) improved semen quality and conception rate and significantly (P<0.01) decreased the amounts of GOT, GPT and ALP enzymes released into the extracellular medium as compared to free caffeine medium, during storage at 50C (Experiment, 2). The percentage of sperm motility and grade of progressive sperm motility were significantly (P<0.01) increased, while the percentages of spermatozoa with coiled tails and spermatozoa swelling were significantly (P<0.01) decreased in the extended buffalo bull spermatozoa either centrifuged or noncentrifuged with Nacitratelactose solutions at levels of 200 and 300 as compared to 50, 100 or 150 mOsmol/L, during incubation at 370C for up to 60 minutes (Experiment, 3).