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العنوان
Morphological and biochemical identification of some maize genotypes /
المؤلف
El-Abady, Magdy Ibraheim Mohamed.
هيئة الاعداد
باحث / مجدى إبراهيم محمد العبادى
مشرف / أحمد نادر السيد عطية
مشرف / سعد أحمد المرسى
مشرف / على السعيد محمد شريف
مشرف / محمود إبراهيم العميرى
الموضوع
Maize. PAGE. SDS. RAPD. Biochemical identification. Morphological identification. Corn. Zea mays.
تاريخ النشر
2004.
عدد الصفحات
170 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
العلوم الزراعية والبيولوجية
تاريخ الإجازة
1/1/2004
مكان الإجازة
جامعة المنصورة - كلية الزراعة - Department of .Agronomy
الفهرس
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Abstract

The present study was carried out in Tag AL­Ezz, Agricultural Research Station Farm, ARC, Dakhlia Governorate during 2003 and 2004 seasons. The objectives of this investigation were to identify six maize genotypes in morphological (quantitative and qualitative) characters. The laboratory experiments were carried out at the laboratory of Seed Technology Research Unit, Dakahlia Governorate and Seed Technology Research Department, Giza Governorate, A.R.C. to study the quantitative and qualitative characters for seed and seedling. Also, protein fractionation and PCR in DNA of seed of maize genotypes. from this study it can be concluded that: 1­ Gm. 2 inbred line was identified by highest numbers of rows/ear and kernel thickness, lowest plant height and ear length, dark green blade color, very strong hairs on margin of sheath, rectilinear attitude of tassel branches, absent anthocyanin of glumes (excluding base), medium anthocyanin coloration of silks, conical ear shape and lowest seedling dry weight. Also Gm. 2 seeds characterized by proteins with molecular weight of 149.630, 110.644 and 69.362 KD. DNA pattern of Gm. 2 using primers B11, B12,B16, B17 and B18 produced 6, 4, 1, 11 and 10 bands, respectively. 2­ Sd. 63 inbred line was characterized among studied genotypes by lowest length of blade of ear­bearing leaf, number of leaves/plant and above the ear, stem diameter, 100­kernel weight and root length. Also, it produced medium hairs on margin of sheath, dark brown silk coloration inside ear. However, it gave the highest electrical conductivity. It had proteins with molecular weight of 166.518, 145.983, 128.639, 120.000, 78.366 and 72.526 KD. DNA pattern of Sd. 63 using primers B11, B12, B17, B18 and B20 produced 7, 13, 18, 19 and 9 bands, respectively. 3­ Sd. 7 inbred line was identified among studied genotypes by highest angles between blade and stem, number of days to 50 % tasseling and 50 % silking, times of anthesis, time of silk emergence and number of days from planting to maturity. However, it produced the lowest number of ears/plant, ear diameter, number of rows/ear, length of peduncle and kernel length. Also, it had faint green blade color, weak hairs on margin of sheath, recurved at one side attitude of tassel branches, white silk coloration inside ear, dent like type of grains and lowest shoot length. Also, Sd. 7 had proteins with molecular weights of 228.169, 165.166, 136.830, 105.268 and 98.773 KD. DNA pattern of Sd. 7 using primers B11, B12, B16 B17 and B18 produced 2, 9, 2, 5 and 4 bands, respectively. 4­ S.C. 10 was characterized among studied genotypes by highest seedling length, number of leaves/plant, plant height excluding tassel, ear height, plant height included tassel, ear length, length of peduncle, kernel width, very strong anthocyanin coloration of glumes, very strong anthocyanin coloration of silks, faint brown silk coloration inside ear, heaviest seedling dry weight. Also, S.C. 10 identified by proteins with molecular weights of 281.646, 258.554, 169.245, 142.449 and 121.572 KD. DNA pattern of S.C. 10 using primers B11, B12, B14, B17 and B18 produced 5, 3, 1, 1 and 3 bands, respectively. 5­ S.C. 21 was identified among studied genotypes by highest length of blade of ear­bearing leaf, number of leaves/plant above the ear, ratio height of insertion of upper ear to plant height, stem diameter, number of tassel branches, length of tassel branches, length of main axis above upper side of tassel, number of ears/plant, ear diameter and cob diameter. Also, it had the lowest number of days to 50 % tasseling and 50 % silking, time of anthesis, rectilinear attitude of tassel branches, weak anthocyanin coloration of silks, dark brown silk coloration inside ear. In addition, S.C. 21 produced the highest shoot and root length. S.C. 21 was identified by proteins with molecular weights of 259.769, 173.383, 152.119, 134.686, 103.793, 100.656, 77.349 and 73.431 KD. DNA pattern of S.C. 21 using primers B12, B14, B16, B17 and B18 produced 3, 1, 6, 9 and 5 bands, respectively. 6­ T.W.C. 321 was characterized among studied genotypes by highest width of ear­bearing leaf, kernel length, 100 kernel weight. It had the lowest kernel width and kernel thickness, faint brown silk coloration inside ear, proteins with molecular weights of 183.226, 155.934, 112.994 and 68.372 KD. DNA pattern of T.W.C. 321 using primers B11, B12, B14, B17 and B18 produced 5, 5, 6, 8 and 1 bands.