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العنوان
Differences between farm animals in (Ivm And Ivf) in vitro maturation and in vitro fertilization /
المؤلف
Rezk, Wael Ahmed Khalil.
هيئة الاعداد
باحث / وائل أحمد خليل رزق
مشرف / مصطفى عبدالحليم الحرايرى
مشرف / عبدالخالق السيد عبدالخالق
مشرف / مصطفى عبدالحليم الحرايرى
مناقش / عبدالخالق السيد عبدالخالقعبدالخالق السيد عبدالخالق
الموضوع
farm animals - vitro maturation. farm animals - vitro fertilization.
تاريخ النشر
2005.
عدد الصفحات
172 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم الحيوان والطب البيطري
تاريخ الإجازة
1/1/2005
مكان الإجازة
جامعة المنصورة - كلية الزراعة - انتاج حيواني
الفهرس
Only 14 pages are availabe for public view

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Abstract

The present study was carried out to investigate the recovery rate, oocyte categories and in vitro maturation of camel oocytes. Two experiments were conducted; the 1st was carried out to study the effects of harvesting technique, reproductive status and site of the ovary on oocytes recovery rate and oocyte categories. Oocytes were collected using two methods, aspiration and slicing techniques. The oocyte yield from each harvested technique was recorded and the number of oocytes/ovary was calculated. Oocytes were evaluated under inverted microscope and classified as follows: Good quality, Fair quality, Poor quality, Denuded, Degenerated and Fragmented. In the 2nd experiment the effects of type of serum added to maturation medium (10% fetal dromedary camel serum or 10% bull camel serum or 6mg/ml bovine serum albumin fraction V) ,reproductive status (pregnant or non­pregnant) and Site of the ovary (right or left) on in vitro maturation of camel oocytes collected by aspiration technique were studied. Only, good and fair oocytes from experiment 1 were used in this experiment. After maturation of the oocytes for 42 h at 3ْ9 C and 5% CO2, they were categorized as follows: Germinal vesicle, Germinal vesicle breakdown, Metaphase­(Qf(B, Metaphase­(Qff (Band Degenerated. The present study indicated the highest recovery rate of total oocytes using slicing technique in collection of camel oocytes from the right of non­pregnant camels. The highest frequency distribution of normal oocytes was observed from the right ovaries of pregnant camels using aspiration technique. The highest maturation rate of oocytes at metaphase­II stage was found on oocytes recovered from the left ovaries of non­pregnant camels by aspiration technique and using fetal dromedary camel serum as a supplement to maturation medium