الفهرس 
Introduction and aim of the thesisOnly 14 pages are availabe for public view
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Abstract
Modern Approaches In Identification And Diagnosis Of Hepatitis C VirusIn the present study: 1Serum samples of 300 individuals, including 256 males and 44 females tested for the RNA using RTPCR. Of 300, 250 cases were positive for HCV<U+2013>RNA using RT<U+2013>PCR (83.3 %). 50 cases (16.7 %) were negative for HCV<U+2013>RNA. The sensitivity and specificity of anti<U+2013>HCV antibody using ELISA and HCV antigen using Dot ELISA were evaluated in comparison with RT<U+2013>PCR technique as a gold standard method. 2The antibody was detected in 240 of 250 HCV<U+2013>RNA positive serum samples with sensitivity 96%. 40 of 50 HCV<U+2013>RNA negative serum samples were negative antibody with specificity 80%. 3 A highly specific and reactive antiHCV antibody was used as a probe in DotELISA to detect a target HCV antigen in serum. This is a semi quantitative assay, requires no sophisticated equipment, rapid (5 minutes) and requires little or no skill to perform without pretreatment of serum sample and the results can be read visually without the need of ELISA reader. An intense violet color was observed in serum samples of HCV infected patients but no reaction with noninfected serum samples. The produced violet color in case of positive serum HCV for antigen detection varied in its intensity from weak (1+,2+) to strong (3+,4+) according to antigen level in serum sample. 4The antigen was detected in 224 of 250 HCV<U+2013>RNA positive serum samples with sensitivity 90%. 46 of 50 HCV<U+2013>RNA negative serum samples were negative antigen with specificity 92%. In conclusion the Dot<U+2013>ELISA for the detection of serum HCV antigen showed high sensitivity (90 %) and specificity (92 %) compared with HCV<U+2013>RNA and could become a useful inexpensive for routine clinical diagnosis of HCV infection.