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العنوان
Studies on Avian Paramyxovirus type-1 in Pigeon /
المؤلف
Ali, Sahar El­-Sayed El-­Sayed.
هيئة الاعداد
باحث / سحر السيد السيد على
مشرف / علي علي إبراهيم القناوي،
مشرف / مختار محمد علي التربيلي،
مشرف / محمد سعيد محمد الشهيدي
الموضوع
Pigeon paramyxovirus type­1. haemagglutination inhibition. agar gel precipitation test. neutralization test. acid. alkaline. ether 20%. experimental inoculation. indirect immunoflourescent technique.
تاريخ النشر
2004.
عدد الصفحات
148 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم الفيروسات
الناشر
تاريخ الإجازة
1/1/2004
مكان الإجازة
جامعة المنصورة - كلية الطب البيطرى - الفيروسات
الفهرس
Only 14 pages are availabe for public view

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Abstract

Hundred pigeon samples were randomly collected from different pigeon lofts in Dakahlia Province. This pigeons suffering from nervous manifestations and diarrhea. Parts from liver, spleen, lung and brain were collected for isolation and identification of pigeon Paramyxovirus type­1 by some serological tests such as haemagglutination inhibition, agar gel precipitation test and neutralization test followed by studying relationship between isolated strain and NDV (Lasota strain). Also, seventy five sera were collected from pigeon suffering from the same clinical signs for detection of antibodies of PPMV­1 physiocochemical studies of the isolated strain and standard PPMV­1 strain were performed by studying effect of 56oC, acid, alkalines and ether 20%. Experimental inoculaion of one of the isolated strains in hundred squabs by different routs (I/V, I/N, I/M and S/C) was performed the reisolation of the inoculated virus in different tissues after different period intervals. We concluded that PPMV­1 could infect pigeons by nervous manifestation and diarrhea as isolation and identification of the virus were performed from these pigeons also antibodies against PPMV­1 were detected in diseased pigeons suffering from the same signs. Also there is antigenically and serologically relationships between isolated virus and lasota strains. PPMV­1 was affected by exposure to 56oC for 30­60 min, acid and alkaline and ether 20%, reisolation and detection of the virus from tissues of experimentally infected squabs by different routes at different period intervals beside indirect immunoflourescent antibodies technique were used for confirming the isolated virus.