الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 199 |  |  |
| | | from | 199 | | |
Abstract
Shiga toxinproducing Escherichia coli (STEC) strains are an important cause of gastrointestinal disease in humans, often with severe complicating problems that include bloody diarrhoea, haemorrhagic colitis (HC), and the lifethreating haemolytic uremic syndrome (HUS). In this study 131 cases (66 females and 65 males) with age ragned from 2 months up to 9 years, were taken from pediatric rehydration center, out patient clinic and inpatient department of pediatric Mansoura University Hospital. Patients diarrheal samples were taken from the patients and inoculated onto selenite broth then subcultured on MacConkey agar. All were incubated aerobically at 37oC for 24 hours. The colonies were identified as follows: The culture character and colony morphology. Gram stained films. from the above results we can concluded that: STEC are currently important human enteropathogen which cause acute bloody diarrhea and may probably developed to urinary tract infection. This study demonstrated that E.coli non O157:H7 had the same prevalence as E.coli O157:H7 in diarrheal samples and more important as E.coli O157: H7 in causing disease. PCR is as sensitive and specific as CTSMAC and serotyping combined. ga shemolysin production on blood agar is more cheep and sensitive as PCR which give approximatley the same results. Bloody diarrhea is more prevalent among infants with artifical and mixed feeding than breast feeding infants. The severity of the diseases seemed also to be related to the type of feeding. Diagnosis of certain types of acute infectious bloody diarrhea, based on clinical data is possible by using the discriminate analysis.