الفهرس | Only 14 pages are availabe for public view |
Abstract In this study we aimed to investigate the effects of H. pylori infection on cell cycle regulation in tumor cells and its relationship to ploidy, proliferation, apoptosis, p53, bcl2, PCNA, clinical and pathological parameters. Therefore, 43 cases with stomach cancer were subjected to flow cytometric analysis including DNAcontent (ploidy) analysis, Proliferative index, percentage of nuclear p53 (by dual parameter flow cytometric analysis). Histopathology analysis including determination of mitotic index and apoptotic index and immunohistochemistry including immunostaining of nuclear p53, PCNA and BCL2 were also done. In conclusion, our results showed incidence of H. pylori in both intestinal and diffuse type gastric cancer. And that gastric tumors <U+2013>irrespective of their type have a significant increase in the cellular Proliferative activity as a compensatory mechanism to counteract DNA damage induced by H. Pylori. These tumors were found to have abnormal DNA content aneuploidy as well as p53 overexpression and increased apoptosis. These data provide supporting evidence of an association between H. pylori infection and gastric cancer and indicate that H. Pylori may have role in the carcinogenesis of gastric cancer through an acceleration of cell kinetics and in the regulation of gastric epithelial cell cycle including increased apoptosis and proliferation, associated with accumulation of p53. Our results suggest that enhanced p53 expression in gastric mucosa may be involved in H. pylorirelated gastric carcinogenesis through accelerated cell turnover. |