الفهرس 
Introduction
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Abstract
Pigeon poxvirus (PPV) is a wide spread disease in Egypt causing high economic losses .In the present study ,trials for isolation pigeon poxvirus from 40 skin ,40 pooled organ (such as lung, liver ,spleen and heart) ,40 red mites and 9 pigeon fly samples collected from infected and freshly dead pigeon from market and pigeon lofts in Dakahlia . These pigeon were suffered from cutaneous lesions. The prepared suspension from different samples was subjected for virus isolation using embryonated chicken egg (ECE) aged 912 days by choroallantoic membrane (CAM) route. Three egg passages were carried out for each sample. The isolated PPV was identified using agar gel precipitation test (AGPT) and reverse Passive haemagglutination test (RPHA).Also samples from naturally infected squabs were collected in 10% formalin for histopathological examination. The haemagglutination ability of the isolated pigeon poxvirus was determined using 1% chicken RBCs .The isolated Pigeon poxvirus was inoculated on CAMs of ECE for detection of virus pathogenesis on these CAMs by histopathological examination using H&E ,Gim(Rb(Benze stain, immunoflorescent and immunoperoxidase techniques .Experimental infection of the isolated pigeon poxvirus (106 EID50) in squabs by scarification to head region and follows the presence of virus in different tissues of experimentally infected squabs using immunoflorescent and immunoperoxidase techniques . Pigeon poxvirus was isolated virus from pigeons suffering from skin lesions and was identified using specific hyperimmune serum .Reverse Passive haemagglutination test is more sensitive test for detection pigeon poxvirus in field samples than agar gel precipitation test.Pigeon poxvirus was isolated from red mites and pigeon fly sample collected from this idicate that red mites and pigeon fly are an important vector for transmission of pigeon poxvirus to pigeon so it must be controlled. PPV have no ability for haemagglutination of 1% chicken RBCs. Detection of virus pathogenesis on CAMs of ECE revealed that PPV can be detected one day post inoculation ((PI) using immunoflorescent` (IF) and immunoperoxidase (IP) antibody techniques but PPV inclusion bodies can be detected two days PI using H&E, Gim(Rb(Benze stains .The isolated PPV was detected again in different tissues of experimentally infected squabs by using IF and IP for their rapid virus detection.