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العنوان
Spectroscopic studies on the radiation induced structural changes in a biological system of whole-­body irradiated experimental animals /
المؤلف
Abd El­-­Razzak, Abd El­-Razek Bedeer.
هيئة الاعداد
باحث / Abd El­-Razek Bedeer Abd El­-­Razzak
مشرف / Mostafa Deiab Migahed
مشرف / Gamal Said El-­Bahy
مشرف / Safaa Khalil Hasan
الموضوع
Gamma radiation. Dielectric spectroscopy. Fluorescence spectroscopy. Hemoglobin.
تاريخ النشر
2005.
عدد الصفحات
124 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الفيزياء وعلم الفلك
تاريخ الإجازة
1/1/2005
مكان الإجازة
جامعة المنصورة - كلية العلوم - Department of Physics
الفهرس
Only 14 pages are availabe for public view

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Abstract

This thesis was devoted to carry out spectroscopic studies on the radiation induced structural changes in a biological system of whole­body irradiated experimental animals. Female rats have been chosen for this study. The rats have been divided into 5 groups A, B, C, D and E. Every group consisted of 5 rats. Group A considered being the control on (unirradiated). Gamma radiation of different doses 1, 2, 4 and 6 Gy was used as ionizing radiation. The rats were exposed to gamma radiation and they were partially anesthetized after one hour of exposure, then blood samples withdrawn and hemoglobin was extracted. Two spectroscopic techniques; namely the optical spectroscopic techniques such as FT­IR, UV/VISIBLE­absorption, fluorescence emission and Dielectric spectroscopy, have been applied to explore the structural changes of the hemoglobin due to gamma irradiation. The recorded FT­IR and UV/Visible spectra reveal the existence of the absorption bands of amides I­IV. The ratio A578/A540 was calculated for all samples and the results revealed that this ratio for all doses is slightly greater than unity indicating that there was no change in the Oxyhemoglobin due to the gamma irradiation.