الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
The present study was undertaken to evaluate the crucial role of hepatocyte transplantation in treating Dgalactosamine induced fulminating hepatic failure (FHF) in rat, in attempt to reach a novel therapeutic strategy, which can help patients supposed to have acute fulminating hepatitis. The rat was used as an animal model to induce fulminating hepatic failure using Dgalactosamine and to isolate hepatocytes from a group of apparently healthy rats. The isolated cells were cultured in vitro and transplanted in FHF rat models either alone or with immunosuppressive. Aim of the work This study aims to evaluate the role of hepatocytes transplantation in treating Dgalactosamine induced fulminat hepatic failure (FHF) in rats. The rat was used as animal model to apply the following lines: (1) Induction of FHF in a group of rats using Dgalactosamine. (2) Isolation and in vitro culture of hepatocytes from a group of apparently healthy rats. (3) Transplantation of the cultured hepatocytes in FHF rat models with and without immmunosuppresive. Results The total yield per liver was 250 x 106 viable cells. The viability was around 90% for hepatocytes isolated by collagenase and 73% for hepatocytes isolated by perchlorate. After culture the viability of the cells was about 90%. In the animals received Dgalactosamine followed by intraperitoneal hepatocytes transplantation and cyclosporine (group V): The survival rate was 80%. The liver functions showed high increase of ALT and AST activities one day after transplantation. Marked improvement of both enzymes was found at day 3. The activities of both enzymes were found near normal at day 7. Changes in liver albumin and prothrombin showed a significant decrease at day 1 then progressively increased at days 3 and 7. Histopathological examination revealed the presence of the transplanted hepatocytes. The cells were tightly attached to the peritoneal covering of the abdominal organs in a single layer but some times more than one layer was found. Binucleated cells were frequently observed. The high survival rate and the improvement of the laboratory findings were highly suggestive of the presence of functioning hepatocytes. Summary and Conclusion Isolation of hepatocytes by enzymatic method was found better that the non enzymatic method. The peritoneal cavity is a promising site for hepatocytes transplantation.