الفهرس 
Introduction and Aim of the WorkOnly 14 pages are availabe for public view
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Abstract
The non Hodgkins lymphomas represent a clinically diverse group of diseases of either Bcell or Tcell origin. EBV is a tumorigenic herpes virus , which is associated with several human hematological neoplasias . EBV induced lymphoproliferative disease represents a broad spectrum , ranging from benign disorders to malignant NHL occurring mainly as a complication of immunodeficiency . This study was conducted on 20 newly diagnosed patients with NHL . Their ages ranged from 15 to 68 years. They were diagnosed through routine investigations (morphology &histopathology ) as well as 5 healthy subjects, with matched age and sex , considered as a control group . Patients were subjected to full history taking , clinical examination , complete blood picture , bone marrow examination and immunophenotyping for peripheral blood , bone marrow aspiration or lymph nodes extraction samples . Serum samples from studied cases and control were subjected also to serological tests for EBV diagnosis by indirect immunoflursence and ELISA techniques.
The purpose of this study is to determine the feasibility of using lymphocytes extracted from lymph nodes, peripheral blood, or bone marrow in lymphoscreen as an initial step in the work up for diagnosis of malignant lymphoproliferative disease in the context of histopathology and further work up in the future, and to correlate that with EBV infection if possible . Patients were classified into 4 groups according to histopathological examination . Their immunophenotyping pattern were as follow: 1 DLBCL (6 patients ):CD19+,CD20+& abnormal k(Sn (Bratio. 2 SLL (4 patients ) : CD19+ , CD20+ , CD23+, CD5+ & abnormal k(Sn (Bratio. 3 Other BNHL (5 Patients ): B.L.(1 Patient ): CD19+,CD20+, CD10+& abnormal k(Sn (Bratio. F.L.(2 Patients): CD19+,CD20+,CD10+& abnormal k(Sn (Bratio. M.C.L. (2 Patients): CD19+,CD20+,CD5+& abnormal k(Sn (Bratio. 4 T NHL (5 Patients): CD3+,CD5+,CD8+. ?EBV detection by ELISA and IF revealed 5 cases were positive by ELISA technique and 7 cases by IF technique . from the present study we can conclude that : Using all displens for diagnosis of NHL is concomitant with histopathological pattern . Association between NHL and EBV . Recommendations : To start the work up of diagnosis of NHL with all known tests : morphology , Immunophenotyping , histopathology, cytogenetics and molecular techniques , and to delineate the role of EBV in NHL using recent molecular approach.