الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 16 |  |  |
| | | from | 16 | | |
Abstract
The present investigation was carried out in the tissue culture Laboratories and the greenhouse of Prof. Helali (In vitro Teach. Lab.) ElMansoura and the Nat. Res. Center, Dokki, Giza, Egypt during the period (19992001). The presented investigation aimed to identify the critical factors affecting on microproduction of banana plant (Musa paradisiaca, L.) Grand Nain?s cultivar throughout all culturing stages of micropropagation. The following factors were examined: 1?Sterilizing agent. 2 Cytokinin (BA) and auxins. 3 Sugars; kind and concentrations. 4 Gelling agents. 5 Activated charcoal. 6Light intensity. 7 Planting media for acclimatization. The main obtained results are summarized as follows: 1?Using the combination treatments of clorox and mercuric chloride or clorox and ethanol reduced the contamination percentages by about 1020% for all explants used. The best sterilization treatment was recorded when mercuric chloride (0.1 g/l) was used for 2 minutes followed by clorox (80 %) for 30 minutes. Such treatment resulted in a complete surface sterilization (85% survival) for all explants used. 2?Cytokinin (BA) at 5 mg/l increase fresh weight, number of shoots and plant height of the explants compared with 2.5 mg/l throughout the duration periods. 3?Multiplication medium containing gelrite at 1.5 and 2 g/l recorded the best results of the fresh weight, shoot number and plant height of the plantlets compared with that medium containing agar at 4 or 6 g/l. Gelrite at 1.5 g/l gave the best result in this respect. 4?Multiplication medium containing sucrose at 40 g/l recorded the best results for fresh weight, shoot number and plant height of the plantlets of banana compared with that contained glucose or fructose. 5? Rooting medium containing MS supplemented with 0.5 mg/l IBM recorded the best results of shoot length and number of leaves of banana plantlet. However rooting medium containing > MS supplemented with 0.5 mg/l IBA recorded the best results for number of roots per plantlets and their length. 6?Rooting medium containing MS salts supplemented with 40 g/l sucrose recorded the highest values of shoot height and root length per plantlet. whereas that supplemented with 20 g/l fructose gave highest numbers of leaves and roots per plantlet. 7?Rooting medium containing MS salts supplemented with 1 g/l activated charcoal gave highest number of roots and their length per plantlet whereas that supplemented with 2 g/l recorded the highest leaf number and the tallest plantlet was noticed with 3 g/l activated charcoal. 8?Plantlet grown under 1000 Lux light intense produced highest values of shoot length, while that grown under 3000 Lux recorded highest numbers of leaves and roots per plantlets as well as their length. 9?Planting medium containing 3 peat : 1 sand (v/v) used at acclimatization stage recorded highest survival percentage and showed vigorously plants having high numbers of leaves and roots as well as plant height. However that grown in medium containing 3 peat : 1 sand (v/v) produced the highest values of root length only. It could be concluded that, using 0.1 g/l mercuric chloride for 2 minutes followed by clorox 80% for 30 minutes was the best treatment for starting stage of banana, Grand Nain cv. For multiplication stage, using MS basal medium + 5 mg/l BA + 1.5 g/l gelrite + 40 g/l sucrose gave high multiplication rate. Furthermore, the best media improved rotting that of > MS basal nutrient medium + 0.5 mg/l IBA + 40 g/l sucrose + 1 g/l activated charcoal and the plantlets incubated in 3000 Lux high intensity. Planting medium contained of 3 peatmoss : 1 sand (v/v) was the best for acclimatization stage.