الفهرس 
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Abstract
The objectives of the research work presented in this thesis were to study the effectiveness of some biological methods in wastewater treatment ; survey of the macrophytes and isolation of the associated fungi in the rhizosphere and rhizoplane of these macrophytes. These plants are growing in both irrigation and drainage canals of Damietta, test the effectiveness of some selected fungi like Aspergillus and Trichoderma to absorb of some heavy metals (e.g. Pb and Cu) and use of some selected macrophytes for the improvement of wastewater quality (e.g. DO; BOD; TP; NO2- ; NO3-- and NH4+) using the aquatic macrophytes in bioremoval of some nutrients, biosorption of some heavy metals by some fungi by two methods. Floristic Composition of Tested Sites Ludwigia stolenifera was the most common plants in three studied areas. Ludwigia stolonifera covered 50-75% in site I, and covered 20-33% in site II, whereas it was most common in site III (75-90%) of the surface area of canals. Pistia stratiotes (Family: Araceae) represented 5% and 20-30%of site II and III. Echinochloa stagnina (Family: Gramineae) covered 5% and 1% of site I and III, respectively Phragmites australis (Family: Gramineae) covered 1% of each of them . Eichhornia crassipes (Family: Pontederiaceae) covered 20-33% of site I. Nymphaea lotus (Family: Nymphaeaceae) covered 2% of sit I. Ceratophyllum demersum (Family: Ceratophyllaceae) covered 5-20% of sit III. Lemna gibba (Family: Lemnaceae) covered 1% of sit III. Sixty-five species were isolated and identified . The fungi isolated were typical of terrestrial origin. The rhizosphere of the selected macrophytes in three studied sites was studied by using plate techniques and direct light microscopy. The majority of species were Mitosporic (48 species) primarily hphomycetes (42), coelomycetes (3) and agonomycetes (3). Ascomycota (9), Zygomycota (6), Basidiomycota (unidentified isolate) and yeast sp. were also isolated. the most common genera were Trichoderma and Aspergillus. The abundant species were Tr. koningii (100% of the samples, 462 colonies) followed by A. niger (90%, 1822 colonies) and A. flavus (90%, 802 colonies).