الفهرس 
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Abstract
Nosocomial infection is the infection acquired in hospital. It is one of
the major problems all over the world conceming the patients and every
member in the hospital. Unless epidemic occurs in hospital, these
infections are of minor or woderate clinical importance.
Laboratory identification of the causative bacteria can be perfonned
by detecting their utilization of carbon and nitrogen through conventional
tube method. Manual identification systems as API may be used with
easier manipulation than tube method, but it does not provide the same day
results. Therefore the use of automated systems are encouraged in many
laboratories for rapid organisms identification and antibiotic sensitivity.
The ann of this work is to identify types of organisms causmg
nosocomial infections with evaluation of environmental role in its
occtUTence at Mansoura University Hospitals. In addition a comparative
study is carried out between the automated system ”Vitek” and manual
methods.
A total of l I 80 samples were collected. They were classified as 465
from patients, 680 from environment and 35 from nurses.
All samples were cultured aerobically on MacConkey and blood agar
plates. Fungal culture was perfonned on Sabauroud’s media. Anaerobic
culture was done for patients samples on Columbia blood agar.