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Abstract The thesis is divided into three parts: Part I.: Including a phytochemical study of Nicotiana glauca that resulted in the isolation and identification of 35 compounds. Part II.: Including a biological evaluation of the different extracts of Nicotiana glauca that resulted in the observation of new antibacterial, antioxidant and anticancer activities Part III.: Including a biotransformation of nicotine by different microorganisms like Absidia pseudocylinder¬ospora and Comamonas testosterone that make a complete degradation of nicotine and Streptomyces griseus that transform nicotine into two new metabolites that were isolated for the first time from a natural source, in this field Absidia pseudocylinder¬ospora and Streptomyces griseus can eliminate nicotine from tobacco solid waste without imposing a hazardous to the environment as they are considered non pathogenic microorganisms. |