الفهرس 
Introduction and aim of workOnly 14 pages are availabe for public view
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Abstract
Meningitis is a common life threatening disease which may be caused by a bacterium, fungus, or virus. Helicobacter pylori bacteria causes gastritis ,duodenal ulcer, and gastric cancer, Here, the presence of a 58 KDa H. pylori antigen were investigated in serum and CSF samples from 173 individuals with meningitis by using SDS-PAGE and Western blotting techniques based on specific anti-H. pylori antibody. SDS PAGE analysis of serum and CSF samples revealed that, a single immunoreactive band only in sera and CSF of H. pylori infected meningitis patients.
Western blotting determined the reactive epitope of H. pylori antigen in both serum and CSF samples of meningitis patients. A sharp reactive band with specific anti-H. pylori antibody was found, the molecular weight of this reactive epitope was determined by a calibration curve represents a relation between the molecular weight of protein standards mixture and their flow rates on SDS-PAGE was constructed showing the molecular weight of standard protein mixture and their flow rates. The flow rate of the reactive band was calculated and its molecular weight was determined from the calibration curve. It was found that the molecular size of the reactive band at an apparent molecular weight of 58 -kDa. ELISA technique was used to detect H. pylori antigen in 84 individuals (49%) of 173 serum samples tested cases and 89 (51 %) of serum samples were negative for H. pylori antigen. But in CSF samples ELISA detected H. pylori antigen in 63 (75 %) of 84 CSF samples showing H. pylori antigen in their sera and 21 (38 %) of CSF samples negative for H. pylori antigen (i.e. of 173 CSF samples, 63 individuals (36%) were positive for H. pylori antigen using ELISA). A significant correlation (r = 0.21, n= 173, p = 0.007,) was shown between serum and CSF levels of 58 kDa H. pylori antigen. In conclusion a 58-kDa H. pylori antigen was identified in serum and CSF samples from meningitis patients. H. pylori antigen was detected in the CSF samples of 75% of meningitis patients showing H. pylori antigen in their sera. The 58-kDa H. pylori antigen cross the blood brain barrier and enter CSF of patients with meningitis. The 58-kDa H. pylori antigen may be of use in immunodiagnosis of meningitis infection.