الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Babesiosis is important haemoprotozoal diseases affecting various kinds of domestic animals and result in a substantial economic losses in several important animal species. In Egypt, Babesia bigemina was widely spread. The main symptom in cattle infected with blood parasite is anemia and haemoglobinurea which may lead to death in severe cases. Fifty four cattle were used for performing the present study their ages ranged from 4 to 6 years for females, from 2 to 3 years for males and from 6 months to 1 year for calves. Microscopic examination using peripheral blood films for detection of babesiosis fail to detect carrier cattle, subclinical babesiosis. PCR is the effective technique for diagnosis of such cases which appeared negative microscopically, but the parasite still in the blood of animals. We developed a PCR-based method for the direct detection of B. bigemina carrier cattle that is superior to existing methods. The method is highly sensitive, is broadly applicable to strains of the parasite from diverse geographic regions, and is specific for B. bigemina rather than the other hemoparasites tests. The sensitivity of this method will facilitate analysis of vaccines and their ability to induce or prevent the carrier stage. Additionally, the method may have use for testing the efficacies of drugs against the parasites and in studies on the transmission and epidemiology of the disease.