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Abstract
1-Ten soil samples were collected from different cultivated and non-cultivated localities in Sharkia (8 samples) and Dakahlia (2 samples) governorates.
2-Such soil samples were subjected to chemical and physical analysis, the highest organic carbon content was recorded for soil sample no. 7 followed by soil sample no. 6.
3-Lipolytic bacteria were isolated and counted by using spread plate technique on a medium supplemented with olive oil emulsion. Mesophilic bacteria were incubated at 30.0˚C while thermophilic ones at 55.0˚C.
4- All the tested ten soil samples contained considerable numbers of lipolytic bacteria, however the highest mesophilic (1.69x106 c.f.u./g soilDW) and thermophilic (2.9x104 c.f.u./g soilDW) lipolytic bacterial populations were counted in soil samples no. 7 (Shrweedah-Sharkia, non-cultivated) and no. 6 (Hehia-Sharkia, cultivated), respectively. Out of 205 lipolytic bacterial isolates were isolated and purified.
5- The isolated lipolytic bacteria (153 mesophilic and 52 thermophilic isolates) were screened for lipolytic activity, by using olive oil-hydrolysis test. Of these isolates, 16 mesophilic and 6 thermophilic had the most lipolytic activity. The most lipolytic ten isolates (7 mesophilic & 3 thermophilic) were selected by using tributyrin-hydrolysis test.