الفهرس 
Arabic Summary
IdentificationOnly 14 pages are availabe for public view
 |  | from | 101 |  |  |
| | | from | 101 | | |
Abstract
current laboratory diagnosis of animal brucellosis based on conventional serological or bacteriological testing procudures is time-consuming and unsatsfactory in several asbects due to lack of rapidity, specificty and sensitivity. Therefore the objective of the present study was to investigate the adaptation of PCR amplification assay as a rapid and sensitive alternative test for the detection of brucella species either following cullure or directly in field samples. A total of 104 serum samples were collected from cows and sheep and subjected to arapid screening test for brucellosis by using RBPT and SAT. The bacteriological examination of milk, lymphenode, uteri and fetal fluid samples reveld the isolation of 6 Br. abortus bv.1, 10 Br.melitensis bv.3 and two isolate not typed species and biovar.