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العنوان
Biochemical studies on vertical transmission of toxoplasma circulating antigen /
المؤلف
El-Mouayed, Mona Abd El-Rahman.
هيئة الاعداد
باحث / منى عبد الرحمان المؤيد
مشرف / احمد م. الوصيف
مشرف / عبد الفتاح م. عطة اللة
مناقش / إبراهيم فتوح حسن
الموضوع
Biochemistry. Toxoplasmosis.
تاريخ النشر
2009.
عدد الصفحات
135 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الكيمياء
تاريخ الإجازة
01/01/2009
مكان الإجازة
جامعة المنصورة - كلية العلوم - Department of chemistry
الفهرس
Only 14 pages are availabe for public view

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Abstract

The detection of toxoplasma-specific antibodies is the primary diagnostic method to determine infection with toxoplasma in pregnant women. The present study aimed to evaluate the vertical transmission of anti-Toxoplasma gondii antibodies (IgM, IgG). ELISA is one of the easiest tests and most helpful technique in the diagnosis of toxoplasmosis. The 36 kDa toxoplasma gondii-(T.gondi) circulating antigen was isolated and purified from serum of toxoplasma infected mothers and umbilical cord samples of their neonates by electroelution. This antigen was used to detect the anti-T.gondii IgM and IgG antibodies in 95 serum samples from pregnant mothers and 95 umbilical cord samples collected from the umbilical cord of their neonates. Total anti-T.gondii IgM antibodies were detected in 32 (33.7%) serum samples with vertical transmission rate (9.4%). Total anti-T.gondii IgG antibodies were detected in 26 (27.4%) serum samples with vertical transmission rate (53.8%). The serodiagnosis of toxoplasmosis based on the detection of antibodies is not useful for the detection of toxoplasma vertical transmission. However, T.gondii circulating antigen was detected in 55 (58%) serum samples with vertical transmission rate (81.8%) in respect to antigen positive. Therefore, the detection of T.gondii circulating antigen in umbilical cord serum is considered as a good evidence for vertical transmission of this antigen.