الفهرس 
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Abstract
Introduction: Babesia is blood parasite infects cattle has an economical important, may be to death and tick-transmitted protozoan parasite. Blood film examination is necessary for identification of Babesia parasite in stained thin and thick blood films as true evidence of infection. However, negative microscopic examination does not exclude the possibility of infection where in early or chronic stages of infection the detection of Babesia in stained blood films is uncommon due to low parasitaemia. So, it is necessary to detect specific antibodies against Babesia species by serological tests rather than Babesia organisms. ELISA is one of the sensitive and specific test used for diagnosis of Babesia.
Aim of the work: the present work is planned to clarify the prevalence of blood parasites affecting cattle in Dakahlia province, Egypt. In this regard, identification of the revealed parasites, their incidences and seasonal dynamics are studied by using blood film examination and ELISA as well as the arthropod vector infestation.
Material and Methods: 336 examined cattle between 3-6 years old located at Sherbeen, El-Senbelawean, El-Mansora, Aga and Belkas in Dakahlia province were investigated in the present study for the presence of Babesia bigemina by use of parasitological (Giemsa stained blood smear) and serologically (Enzyme linked immunosorbent assay, ”ELISA”) methods.
Results: The present investigation revealed that 24 (7.14%) out of 336 cattle by Giemsa stained blood smears were infected with Babesia bigemina. 336 cattle serum samples in the present investigation by ELISA showed 78 (23.2%) infected with Babesia bigemina. The seasonal incidence of Babesia bigemina revealed that highest incidence of Babesia bigemina in Dakahlia province was 10.3% in summer followed by 7.7% in autumn while during winter, spring was 0 % and 6.1% respectively. But in summer 34.5% and autumn 32.1% while 8.3% and 9.8% in winter and spring respectively by ELISA. The incidence of Boophilus annulatus tick out of 336 examined animals in Dakahlia province was 7.14%.