الفهرس 
Antibacterial effect of dental adhesivesOnly 14 pages are availabe for public view
 |  | from | 137 |  |  |
| | | from | 137 | | |
Abstract
Introduction: Secondary caries is the major factor that influences the longevity of dental restorations, particularly when composite restorations are placed with the cervical cavity margin in dentin. Hence, attention has been paid to the therapeutic effects revealed by direct filling materials. One of these effects is the antibacterial potential, because inactivation of bacteria means a direct strategy to eradicate the cause of dental caries. Aim of the study: This study was aimed to evaluate the anti-cariogenic potential of four different types of dental adhesive systems namely: MDPB, fluoride and glutaraldehyde containing adhesive systems, and a single component adhesive system as a control type, to study the cytotoxic effect of the same dental adhesive systems on cultured human pulp fibroblasts and to clinically evaluate the effectiveness of the studied dental adhesive systems. Materials and methods A total of eighty human upper premolars, free of caries and other defects was used in this study. The roots were cut off and the pulp tissues were removed. For each tooth, a standardized cervical cavity was prepared on the buccal surface. The cavities of each group were restored with one of the tested adhesives followed by incremental placing of one type of hybrid resin composite. The specimens were then incubated in an artificial caries chamber for four weeks. Longitudinal sectioning of the specimens was done into two halves: the first half was examined for bacterial leakage using bacteria detecting stain (Brown& Brenn stain), and the second half was examined for demineralized areas using a laser fluorescence device and validation of the readings was done using the fluorescence microscopy . Cytotoxicity of the adhesive systems was evaluated after immediate exposure of pulp fibroblast cells to media extracted from cured and non-cured adhesives. Clinical evaluation 60 restorations placed in 15 patients. Each patient had at least four cervical moderate non-exposed carious lesions. The restorations were assessed for postoperative sensitivity, recurrent caries, marginal discoloration and retention according to USPHS modified Ryge criteria at baseline, six months and 12 months after restoration placement. Results: The results of the present study revealed no significant difference in caries inhibitory effect induced by different adhesive systems. All tested adhesives represented a cytotoxic effect to pulp fibroblast cells. Clinically no significant difference between between studied adhesives. conclusions: 1- Incorporation of antibacterial components in the dental adhesive systems could not inhibit bacterial ingress through tooth-restoration interface which may be correlated to the doubtful sealing ability of the adhesive systems. 2- The use of MDPB containing adhesive system has resulted in more caries inhibitory effect if compared to fluoride containing and glutaraldehyde containing adhesive systems. 3- Addition of the antibacterial components in the dental adhesive systems could adversely affect the biocompatibility of these adhesives when coming into contact with vital pulp cells. 4- The clinical behavior of the tested adhesives in moderate depth cavities after one year was even regardless of their in vitro cytotoxic effect.