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العنوان
Micropropagation studies on somatic regeneration of wheat plant under salt stress /
المؤلف
Arafa, Sally Arafa Ahmed.
هيئة الاعداد
باحث / sally arafa ahmed arafa
مشرف / mohamed nasr helali
مشرف / mamdouh mohamed abd el-maksoud
مشرف / zein el-abedin abd el-hameid mohamed
الموضوع
salinity. wheat genotypes. tissue culture technique. plantlets. callus.
تاريخ النشر
2010.
عدد الصفحات
146 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم النبات
تاريخ الإجازة
1/1/2010
مكان الإجازة
جامعة المنصورة - كلية الزراعة - agricultural botany
الفهرس
Only 14 pages are availabe for public view

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from 163

Abstract

Studied the ability of 8 genotypes of wheat to tolerate or acclimatize NaCl salt stress condition using tissue culture technique at the laboratories biotechnology and Agriculture Botany , Faculty of Agric. Mansoura Univ., Egypt during the period elapsed from 2007-2009. The obtained results could be summarized as follows: I.Seed germination and seedling growth: 1. Salinity decreased germination percentage and its rate of all tested genotypes . Moreover, the growth rate of the seedling was also decreased except with Gemmeiza 7 and Gemmeiza 10x C.B.255. 2. Salinity increased the concentrations of proline and total sugars as well as sodium whereas, decreased potassium. II. Micropropagation: 1. Salinity decreased fresh and dry weights of the embryogenic callus of all investigated wheat genotypes and deceased chl a , b concentrations in the plantlets except that of both genotypes Gemmeiza 7 and Gemmeiza 10x C.B.255 which showed an ability to tolerate salinity. 2. Total sugars and free proline concentrations in the embryogenic wheat calli were increased due to salinity . Moreover, changed the hormonal levels and their balances within the tissues. 3. The anatomical investigation showed certain modification in the structure and tissue organization in the root and leaves of in vitro plantlets. In addition, number of stomata and prickles were decreased on the leaf surfaces. Microhairs were differentiated and are supposed to be secretary glands to help plants to alleviate salt conditions. 4. Growth parameters studied of the regenerated plantlets were adversely affected by salinity. Moreover, Na+ was increased whereas, K+ decreased. Conclusion: It could be concluded that, genotypes Gemmeiza 7 and Gemmeiza 10xC.B.255 were the most tolerant to NaCl stress as compared to other investigated genotypes under the present investigation. Tissue culture technique can be used safety for propagation of wheat to produce new lines representing materials needed further investigation.