الفهرس 
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Abstract
Rift Valley fever (RVF) is an arthropod borne zoonotic viral disease emerges periodically throughout Africa, emphasizing that it poses a major threat for animals and humans. A total of 240 blood samples were collected from human and tested for RVFV IgG antibodies by Enzyme Linked Immunosorbent Assay (ELISA) and Serum Neutralization Test (SNT). Four hundred eighty eight blood samples were taken from different animal species and assessed for RVFV IgG antibodies by ELISA, SNT and Fluorescent Antibody technique (FAT). Thirteen mosquito pools (9 Culex species, 3 Aedes species and 1 Anopheles species) were collected by using CDC gravid and subjected to Double Sandwich ELISA for detection of RVF antigen. Trial for virus isolation through intracerebral inoculation (I/C) in suckling mice was carried out. Confirmation of Double Sandwich ELISA results by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) was done. The overall occurrence of RVFV IgG antibodies in man was 19.6%. Overall percentage of RVFV IgG antibodies in male was 22 but in female was 16.8. Highest occurrence of RVF antibodies was in age groups 51-60 years. Higher percentages of RVF antibodies were found in sera collected during winter seasons, in sera from occupations having animal contacts especially shepherds and in sera of people residing rural areas. Overall percentage of RVFV IgG antibodies in examined animals is 31.8%. Vaccinated cattle showed higher percentages indicating the role of vaccination in protection. Previously aborted animals had higher percentages of RVF antibodies. One Culex pool showed positive result by Double Sandwich ELISA. Trial for virus isolation was failed. RT-PCR confirmed positive result of Double Sandwich ELISA. It could be concluded that surveillance system must be alert for any expecting outbreak from neighboring countries or flaring up infection during inter-epizootic period.