الفهرس 
Introduction
Physicochemical characteristics of water sampleOnly 14 pages are availabe for public view
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Abstract
Water monitoring of El-Lessan area was conducted during four seasons of one year, 2008, indicates high level of contamination with petroleum hydrocarbons and poly cyclic aromatic hydrocarbons. where, these pollutants have acute and chronic toxicity and tumorigenic effect on human health and other living organisms. Therefore, as a suggestive solution for this highly polluted area, fifty different bacterial strains, three yeast and nine fungi are isolated from the highest oil polluted site in El-lessan area for their ability to uptake and metabolize 2,2’- BHBP. Different tests and screening techniques were used for selection of most tolerant BDM, which revealed that the four most predominant and potent isolates with highest growth potential and metabolic capabilities on different Poly aromatic compounds labeled; Sh1, Sh15, Sh42 and ShF4 and identification according to 16S r-DNA sequencing for bacterial strain and microscopic morphology of fungal strain as Micrococcus luteus, Corynebacterium accolens, Corynebacterium variabilis and Asperagillus flavus, respectively. GC-MS analysis was done to investigate metabolic pathways of 2,2’-BHBP, 2-HBP, Pyrene and DBT as model for phenolic, PAHs and PASHs compounds, respectively using C. variabilis sp. Sh42 revealed that, all studied compounds were completely metabolized using different mono- and dioxygenases enzymes for production of energy source (Adenosine tri-phosphate, ATP) needed for expression of enzymes required for growth and metabolic pathways. The dead end by-product of all pathways is pyruvic acid. As a suggestive solution to minimize and treat oil pollution in El-Lessan area; the capability of C. variabilis sp. Sh42 for bioremediation and cleaning up of oily polluted water collected from El-Lessan area individually and in consortium with the reference bacterium species Rodococcus erythropolis IGTS8 were studied. Follow up the effectiveness of biotreatment processes by different analytical techniques indicated that C. variabilis sp. Sh42 has highest capabilities to bioremediat different hydrocarbons i.e. aromatic, hetero- aromatic and aliphatic compounds which were completely metabolized after 28 d.