الفهرس | Only 14 pages are availabe for public view |
Abstract Salmonella is one of the most important pathogenic bacteria in the world that can infect chicken causing serious illness in human through consumption of contaminated food products as there are more than 2800 serotypes that can infect chicken. In the present study of Salmonella infection at Dakahlia Governorate on 152 chicken samples in two seasons cold (winter and spring) and hot (summer and autumn) seasons and the results were summarized as the follows: 1. Salmonellae were isolated from (27) out of (152) chickens that were examined (17.8%) with high incidence during hot seasons (25%) other than in the other seasons (10.5%). 2. The rate of recovery of Salmonellae from the different internal organs showed that high recovery rate was from liver, caecum, spleen and then small intestine as the follow (12.5%), (8.55%), (7.24%) and (3.29%), respectively. 3. The results of serological serotyping revealed that Salmonella Typhimurium 13.8% (21 isolates) and Salmonella Kentucky 3.95% (6 isolates) from the examined samples. 4. S.Typhimurium and S.Kentucky were isolated from livers while S.Typhimurium is the only serovare which isolated from small intestine, caecum and spleen. 5. PCR-polyacrylamide gel electrophoresis detect flagellar gene of Salmonella Typhimurium with amplicon of 375 bp. 6. S.Typhimurium has the ability to form biofilms on glass surface of the test tubes. 7. Salmonella Typhimurium and Salmonella Pullorum were found to be sensitive to chloramphenicol, gentamycin and ciprofloxacin and were resistant to flumequine, lincomycin, nalidixic acid and oxolinic acid and so proved that there is no correlation between biofilm formation by flagellae and antibiotic susceptibility. |