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Abstract Lipases as biocatalysts havc a great intorcst in hts and oils processing and other rclatcd industries such as drugs; flavoring agents; emulsifiers; cosmetics and deterzcnts. Lipases have highest potentiality due to the reaction selectivity and mild reaction conditions. Hence this study was carried out to produce microbial lipases using two strains of fungi nan~elyR, hizopus arrhizus DSM 906 and Rhzzopu~. oligosporus DSM 1964. The study included also the effect of vaxious nutritional and environmental hctoss on growth and lipase production by the two fungal strains. ’l’he produced lipases were isolated, immobilized and characterized. ’h enzymes were industrially applied in reducing free fatty acids in some crudc oils. ’I’he following results were obtained: 5.1. Production of lipases: 5.1.1. N-u- -t ritional factors: 5.1.1 .l. Glucose was found to be the best carbon source at concentration of 5% for maxin~unig rowth and at- 1% concentration for maximum lipase production by Rhizops arrhzzus fbllowed by maltose and then stcuch. Fructose and galaclose gave minimum growth and lipasc yield. Also, glucose was found to be the best carbon source at concentration of 5% for Rhizopz~s oligosporus growth while nmxilnum lipase production was obtained with galactose at 1% concentration. 5.1.1.2. Various lipid materials were added to the growth media as lipase inducer. ’The highest growth and lipase production by Rhizopus |