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Abstract t h i s work was directed to study the cottonseed proteins i n view of several points and the resulta were summarized as follows : 1- Three methods were used f o r the preparation of cottonseed f l o u r from d i f f e r e n t v a r i e t i e s of cottonseeds . The f l o u r was prepared by e x t r a c t i n g t h e seeds with e i t h e r acetone, hexane or both solvents, r e s u l t i n g i n products with 53 %, 60.9 % and 62.3 % protein content, respectively. 2- The chemical composition of d i f f e r e n t v a r i e t i e s of deh u l l e d cottonseed and f l o u r prepared with the hexanc._- acetone,acetone-water and acetone method was determined. 3- The cottonseed proteins were extracted from cottonseed f l o u r , prepared with hexane-acetone, with water and urea solutions of incraasing molarity at temperatures of 20 - 40•‹C. A method was developed i n which the $lou:r was extracted with water and 6 M urea, with t h i s method a yield of 87.26% of the t o t a l proteins can be obtained. 4- The cottomeed protein was prepared from cottonseed f l o u r with various methods using water urea s o l u t i o n of increasing molarity (1, 2, 4 and 6 M) at temperatures of 20-40•‹C and various concentration of sodium and calcium chloride. |