الفهرس 
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Abstract
the present study was to manipulate some essential growth conditions of the unicellular green alga C. reinhardtii to maximize i t s photosynthetic capacity, as reflected i n oxygen-evolution rate, protein content and productivity. These conditions included buffer type, carbon source (acetate, CO2 or presence of both), nitrogen source (ammonium chloride, peptone and urea), l i g h t regime and l i g h t quality. This investigation started by using mixotrophic growth i n acetate (KA) since it i s easier and less expensive compared to autotrophic one. I n mixotrophic cells, compared to autotrophic ones, photosynthetic capacity, protein content, productivity were increased and starch was decreased by increasing NH4+/K+ r a t i o and phosphate concentration. Because of the highly uneven distribution of l i g h t energy, suggesting temporary dislink of the two photosystems, under continuous l i g h t , the light-dark cycles were preferred for mixotrophic growth. Each of blue and red l i g h t decreased photosynthetic capacity of mixotrophic c e l l s compared to white l i g h t . Blue l i g h t reduced productivity and increased protein content, while red l i g h t increased starch formation which was associated with l i g h t energy distribution favouring PSI suggesting increased cyclic phosphorylation.