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Abstract 1. This work began as an endeavour t o isolate different strains of Azotobacter spp. from different cultivated Egyptian l o c a l i t i e s . Twenty-one different pure organisms were isolated from these l o c a l i t i e s . 2. Among the isolated organisms , strains No. 3 , 9 and 15 were found to produce varying amounts of riboflavin . 3. A culture medium , formulated a f t e r Burk and Lineveaver (1931) was found t o be the most convenient culture medium for the biosynthesis of riboflavin . 4. Since organism No. 15 could form a considerable amount of riboflavin i n addition t o the highest value of t o t a l nitrogen when cultivated i n medium I1 , it was chosen as the experimental organism . 5. When the various schemes of identification of bacteria were applied on organism No. 15 , it was characterized as Azotobacter chroococcum . |