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Abstract
Millions, of people in subtropical countries arc infected by Schistosoma mansoni, the most common human schistosome (Henri et al., 2002).
Schistosomiasis has been successfully controlled with the use of chemotherapy-based programmes in a number of countries, including Egypt. However despite this success, schistosomiasis unfortunately remains a major public health problem, particularly in Africa (Hagan et al., 2004).
The control of schistosomiasis in many parts of Egypt demands the detection of individuals with low egg counts. The most effective and practical method for diagnosing human schistosomiasis is based on the detection of infection specific antibodies (Tsang, 2000).
The aim of this study was to determine the best schistosomal adult worm antigenic fraction(s) for serodiagnosis of active intestinal Schistosomiasis and to determine whether this antigenic fraction(s) could differentiate patients with active disease from formally treated patients.
This study included 3 groups of subjects. The first group included 10 active egg passers, their age ranged from 10-19 years. Those patients were examined for the first time at 1997 by schistosomiasis research project (SRP project) at Al Azhar University, Faculty of Medicine for Girls Based on clinical and abdominal ultrasonographic examination, patients of this group were: 3 intestinal schistosomiasis patients (had no organomegaly), and 7 hepatointestinal schistosomiasis patients had organomegaly (hepatosplenomegaly). The second group included 10 formally treated patients. Their age ranged from 9-21 years. They were received praziquantal treatment in the dose recommended by the Egyptian Ministry of Health (20 mg/kg body weight) at that time they were not passing eggs in the stools. Ten age and sex matched control subjects were also included.
First, characterization of S. mansoni soluble worm antigen (SWAP) by SDS-PAGE was performed. Followed by Western blot profile analys using active and formally treated patient’s sera.