الفهرس | Only 14 pages are availabe for public view |
Abstract 1, An en?yme inuiiunoassay (EIAJ for human transferrin was established using monoclonal antibodies. 2. Three monoclonal antibodies (MAb 1, 2, and 3) were produced against human cransfprrin. MAb 1 and MAb 2 were characterized to be. of the Ig Gl subclass, while MAb 3 was of thG Ig G 2 3. Nona of the three monoclonal antibodies produced a precipitation art with humnn ti’ansJrerrin on a mlcro^hichterlony plate, Also, a combinai-ifjii of cvo or three Logether did not produce a precipitation lino. The polyclcm.-jl antibodies showed a precip-iLatlon^rc with 4. The Ti^nucloncil Antibodies did not cross react with either rat nr the dog transferrLn. the highest activity to traiisforrin at a dilution of 1 ^j. MAI? ! x 10 ” and was used to develop the ft] A. 6. It w;is not possible to establish the EIA using various combinations ol the three monoclonal ant:bodJes for the solid and indicator antibody. Polyclonei] antibodies were used as the solLJ and LOU jugated MAb 1 as the indicator Antibody. |