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العنوان
MOLECULAR TAXONOMIC STUDIES ON SOME WILD AND CULTIVATED TAXA IN EGYPT
الناشر
Ain Shams.Science.Botany
المؤلف
Hussein,AlSafa Hassan Mohamed
هيئة الاعداد
باحث / السقا حسن محمد حسين
مشرف / سيد فرج خليفة
مشرف / احمد بهي الدين محمد
مشرف / مجدي محمد مراد
تاريخ النشر
2004
عدد الصفحات
220
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم النبات
تاريخ الإجازة
1/1/2004
مكان الإجازة
جامعة عين شمس - كلية العلوم - النبات
الفهرس
Only 14 pages are availabe for public view

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Abstract

The Solanaceae is one of the most important and large families of angiosperms. The largest genus of the family is Solanum, which comprises alone more than half the number of the species. One objective of this research is to study the genetic distance among eight Solanum taxa (three cultivars and five species) on the basis of information derived from macromorphological, micromorphological and seed scan characters as well as criteria revealed by SDS-PAGE of seed storage proteins, RAPD-PCR and specific PCR with an ?-amylase gene family. SDS protein profiles indicated the occurrence of some unique bands, in which some of them were positive, while the others were negative. RAPD analysis was done utilizing fifteen 10-mer random primers and specific PCR with ?-amylase was applied utilizing six pairs of ?-amylase primers and both resulted in the occurrence of some molecular genetic markers discriminating the eight taxa.