الفهرس 
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Abstract
In the present study- is essentially- a follow up of the histopathological, cytological and histochemical changes which are induced in the cerebellum and the spinal cord tissues and cells of white rats under the effect of the narcotic dnlg (diazepam), using both light and electron microscopy.
To achieve this goal, intramuscular injection of diazepam was carried out at the dose levels of 1/4 LD50 for 10 and 36 successive days, 1/2 LD50 for 10 and 30 consecutive days and 3/4 LD50 for 10 and 15 following days.
The results obtained from these experiments are smnmarised as follows:
Light microscopy:
An obvious oedema, congestion and haemorrhage of the submeningeal blood vessels were detected. Purkinje cells and motoneurons became deterioratbd. Many degenerated foci were observed in the white matter of the cerebellum.
The cytological changes:
Nissl bodies were changed into discrete granules and a few small flakes in the motoneurons of the spinal cord. Also, a variety of changes have been observed to occur in the Golgi complex of rat Purkinje cells as well as that of the spinal cord motoneurons after treatment with diazepam.
Treatment with diazepam had produced gradual loss of carbohydrate contents and total protein from both Purkinje in the cerebellmll and motoneuron cells in the spinal cord.
Electron microscopy:
The administration of diazepam had resulted more abnormalities compared with the control animals in Purkinje and motoneurons cells respectively. These abnormalities included, mitochondrial degeneration comprising swelling, separation of cristae, vacuolization and deposition of electron dense material within the the mitochondrial matrices.
The Golgi network became fragmented into small separate elements.
Degeneration of the rough endoplasmic reticulmll was also clearly encOlmtered in the treated cells.