الفهرس 
Material and methodsOnly 14 pages are availabe for public view
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Abstract
Summary
SUMMARY This study was conducted in Clinical Parasitology Department, Benha
Faculty of Medicine, Zagazig University.
The aim of this study was the evaluation of some immunological assays used in diagnosis of human fascioliasis, therefore, we selected our patients upon the definitive diagnostic tool known here which is the positive stool analysis for Fasciola eggs and considered it the gold
standard against it we evaluated the other techniques.
The study was performed on:
- Thirty Fasciola positive patients (8 males and 22 females with age
ranged between 5 — 56 years old).
- Twenty patients infected with other parasites rather than Fasciola
served as the control positive group.
- Twenty healthy individuals free of parasitic infections served as the
control negative group.
Cases included were subjected to four studies:
- Clinical study.
- Parasitological study. - Hematological study. - Immnuological study.
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Summary
Clinically, the patients were assessed for clinical manifestations suggesting fascioliasis infection, we observed that abdominal pain, anorexia and loss of weight were the major presenting symptoms among those patients.
Parasitologically, we classified the patients using Kato thick smear technique according to the intensity of infection (eggs count) to:
- Mild infection group (40 — 100 eggs/gm): Six patients (20%).
- Moderate infection group (120 — 380 eggs/gin): 15 patients (50%).
- Heavy infection group (400 — 560 eggs/gm): Nine patients (30%) Hematologically, it was found that the mean hemoglobin value of fascioliasis patients was significantly lower than that of control groups, moreover, a significant negative correlation was found between Hb values and eggs count of fascioliasis patients.
In contrast, the mean absolute eosinophilic count of fascioliasis patients was significantly higher than that of control groups.
The immunological study included two issues:
- Antibody detection assays.
- Antigen detection assay.
Firstly, we evaluate the available commercial kit for serological diagnosis of fascioliasis (IHA), this technique proved a sensitivity of 73% and 92% specificity.
153
Summary
Using indirect ELISA technique the four immunoglobulins assays were run; IgG, IgM, IgE and IgA anti Fasciola antibodies.
Indirect ELISA technique for detection of anti Fasciola IgG antibodies diagnosed 28 patients out of 30 Fasciola egg positive cases (93%). The specificity of that assay was 80%.
Using indirect ELISA technique, anti Fasciola IgM antibodies were detected in 25 patients out of 30 Fasciola positive patients (83%), this technique proved a high specificity (90%).
Anti Fasciola IgE antibodies were detected in 10 patients only out of 30 Fasciola egg positive cases (33%), an excellent specificity was proved by
this assay (100%).
Anti Fasciola IgA antibodies were detected in 21 patients out of 30 Fasciola egg positive cases (70%), the least specificity was found with
that assay (72.5%).
Using competitive inhibition ELISA technique, six cases only (20%) were found to have Fasciola antigens in their sera, however, a high specificity for that assay was found (92.5%).
Both of anti Fasciola IgG and IgM antibodies were detected in all
Fasciola antigen positive cases.
The highest specificity for Fasciola diagnosis assays was that of IgE ELISA assay. The least was that of IgA ELISA assay for anti Fasciola
antibodies.
154
Summary
The highest sensitivity for Fasciola diagnosis assays was that of IgG ELISA assay. The least was that of competitive inhibition ELISA assay for Fasciola antigen detection.
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