الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
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Abstract
1. This study was carried out in Alexandria, A.H.Egypt over one year period, from 1st April 1980 to the end of March 1981. 2. The aims were: to isolate and identify halophilic vibrios from seafoods and sea water; and possibly from fresh canal water and sewage; and to find out any possible role of the pathogenic strains in caus . ing diarrhoea. 3. The examined samples were; 382 from seafoods (Raw and cooked) of different varieties, collected from 4 fish markets; 190 from sea water collected from 5 different beaches; 114 from fresh water; and 190 from sewage. The samples were collected once monthly during cold months (November - March) and twice monthly during warm months (April - October). 4. The examined samples also included 2200 stools from healthy food-handlers and 1500 from patients with diarrhoea after consumption of seafoods. 5. All procedures followed in this study were those re commended by Food and Drug Administration, Bacterio logical Analytical Manual (1976) and Guidelines for Health Related Monitoring of Cost~l Water Quality (1977). 6. A total of 641 (14.0 ) halophilic vibrio strains were isolated from 4576 samples collected during this study. (226 v. parahaemolyticus and 415 v. alginolyticus). 7. v. parahaemolyticus and v. alginolyticus were isolated respectively from; 40.6 and 74.6 of seafood sEl.mples; 15.8 and 60 of sea water samples; 1.6 and 0 of sewage samples; 1.8 and 0 of fresh water samples; . 0.6 &nd 0.7 of normal stools, and 1.5 and 0 of diarrhoeal stools. 8. Out of 304 raw seafood samples, 44.1 and 82.6 were found positive for v. parahaemolyticus and v. algino lyticus respectively. Shellfish was found to harbour the highest percentage of v. parahaemolyticus (60.5 ) and v. alginolyticus (94.7 ). Also out of 78 cooked seafood samples; 26.9 and 43.6 were found positive for both organisms respectively. Crab showed the high est isolation rates (50 and 70 ) of both organisms. 9. The highest incidence of v. parahaemolyticus in examin ed samples of fish was found in Bulti (66.7 ) and Barbooni (50.0 ); and of shellfish, was found in Gandofli (72.0 ) and Om-Koloul (64.0 ). 10. Abu-Kir fish market showed the highest isolation rates of v. parahaemolyticus and v. alginolyticus (66.3 and 91.6 ) of examined seafood samples. - ll. Higher isolation rates of halophilic vibrios were found in sea water samples from Anfoushi, Sporting and Gleem beaches (18.4 to 26.3 for v. parahaemolyticus and 71.1 to 84.2 for v. alginolyticus)th&n from Maamoura beach (5.3 and 23.7). The counts of y. parahaemolyticus in the sea water samples examined by membrane filter technique were found to range from 30 to l60/l00ml. in Anfoushi, Gleem and Sproting beaches and from 20 - 40 /lOOml in Maamoura. 12. Out of 38 samples, from each of Smouha open drain sewage and Mahmoudia canal water; 3 (7.9 ) and 2 (5.3 were found positive for v. parahaemo1yticus. 13. Stools of cooks engaged in seafoods showed higher isolation rates of v. parahaen~lyticus and v. algino lyticus (2.3 and 3.9 ) than other food-handlers (0.5 and 0.7 ). 14. Kanagawa test, which is used to demonstrate patho genicity of halophilic vibrios, was positive only in 29 (12.8 ) of v. parahaemolyticus isolates. Of these, (21) were isolated from diarrhoeal stools, (7) from normal stools of food handlers and (1) from seafoods. - .itS? 15. Serotypes 02 and 05 were the commonest among the iso lated:L. parahaemolyticus strains (19.9 E4nd 19.5 ); and 19 were non-typable by the aVE4ilable 11 ”0” antisera for V.’ parahaemolyticus. 16. A seasonal trend, in rates of isolation of halophilic vibrios from sea water, seafoods and stools was observ ed during this study, being higher during warm months than in cold months. 17. The mean value of the M.P.N. of V. parahaemolyticus of raw seafood in this study exceeded the accepted standard count for hygienic safety of seafoods. 18. Two localised food poisoning outbreaks, in Abu-Kir, were investigated; and ~ parahaemolyticus was confirmed to be the causative organism.