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Abstract
The investigation aims to study the hepatoprotective, anti-tumor, antioxidant, antibacterial and antifungal activities of total saponin mulberry root bark and its toxicity effect. In addition to that, separation and identification of new triterpenoid saponins using spectroscopic methods (UV, IR, FAB-MS and 1H NMR) and their biological activities were investigated.
The results of the present investigation could be summarized in the following :- The results of the present investigation are summarized in the following :-
1) The yield of total saponin (TS) was 0.5% of dry plant. While, sapognin was recorded 24.5% of TS as betulin. Sugar moiety of TS was consisted mainly from fructose, galactose, glucose and rhamnose.
2) The pre- and post-treatment with TS protected liver from sever toxic effect and histopathological changes due to CCl4-hepatotoxicity.
3) Antitumor activity of TS was recorded 90% using Erlish cells.
4) Antioxidant activity of TS at a concentration 200ppm was 75.7% comparing with control after 8days of oxidation.
5) TS at different concentrations (250, 500, 1000 and 2000μg/ml) had moderate activity against Bacillus subtilis, Pseudomonas sp. and Proteus vulgaris. Also, it had weak activity against Eschericha coli and Streptomyces sp. and no effect against Erwinia amylovora was observed.
6) TS at different concentrations (250, 500, 1000 and 2000μg/ml) reduced the linear growth of Rhizoctonia solani, Sclerotium rolfsii, Fusarium oxysporum, Botrytis cinerea and Alternaria alternata. The highest effect was observed in case of S. rolfsii and B. cinerea. This effect proved by biochemical, electrophoresis and SEM studies. Also, greenhouse experiment proved that TS as a natural plant substance could be used for root-rot disease control induced by S. rolfsii and promoted some growth parameters in tomato seedlings.