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Abstract
Bone and joint infections resulting from bacterial infection either primary or post operatively are common problems facing orthopedic surgeons. Bone, which usually are well protected from infection, can become infected through three routes: the bloodstream, direct invasion, and adjacent soft tissue infections (Nicolau et al., 1998). The bacteria most likely to cause infection depend on a person’s age. Staphylococci, Hemophilus influenza, and some other gram-negative bacilli most often infect babies and young children, whereas gonococci (bacteria that cause gonorrhea), staphylococci, and streptococci most often infect ’older children and adults. Other organisms are seen, including Salmonella (especially in systemic connective tissue disease and sickle cell disease), E. coli and other gram negative bacteria. (Wilber, 1984 and mousa, 2003). this work aimed at:
1- Isolation and identification of bacterial isolates recovered from patients suffering from bone and joint infections in Tanta hospitals.
2- Phenotypic and genotypic characterization of the recovered isolates.
3- Investigation of antibiotic resistance profile and possible resistance mechanisms involved among the selected isolates.
4- Studying the virulence mechanism of selected isolates.
Materials and methods:
A total of 103 specimens were collected from patients suffering from bone infection.
The period of study ran from September 2002 to January 2007. These patients were admitted to department of orthopeadic Tanta University hospital and their ages ranged between 22 days and 86 years.
Conclusions:
The highest incidence (59.2%) of bone infection was observed among cases from 21 years ¬to 40 years old. Bone infection was more common in males (67.96%) than females (32.04%) cases. Out of the 103 studied bone infection cases 86 (83.5 %) showed bacterial infection while others were bacteriologically free. A total of 144 bacterial isolates were recovered from 86 infected cases. Out of these isolates 123 (85.4%) were aerobic and 21 (14.6%) were anaerobic. All the recovered 123 aerobic isolates were subjected to microscopical and biochemical identification. The obtained results showed that 90 isolates were gram +ve cocci while the remaining 33 isolates were gram -ve bacilli. Cultural and microscopical examination of the recovered gram +ve cocci revealed that all these isolates belonged.