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Abstract
The visible light radiation could have an effect on the human eye. The type and degree of damage are related to the duration and the cumulative exposure to the visible light radiation as well as to the intensity of the rays where, the higher the intensity the more damage to the eye. The high energy segment of the visible region (Blue, 400-500 nm) is enormously more hazardous than the low energy portion from 500-700 nm (Green-Red), where the photochemical damage occurs at the border between the perceived colors of green and blue.
A fourty healthy mature chinchilla rabbits were used for the present study that were purchased from the holding company for biological products and vaccine, Cairo, Egypt, and were fed with balanced diet. The rabbits were housed individually in plastic boxes kept in shielded chamber to reduce the normal ambient environmental electric field. Animals were categorized into four main classes A, B, C and D. Animals of class A (10 rabbits) didn’t receive any radiation and kept as control. Animals from the other groups were dark adapted over night; group B received blue light radiation of wavelength (400-500nm), group C received green light radiation of wavelength (500-600nm), group D received red light radiation of wavelength (600-700nm). Each class was then subdivided into two groups (one received low lux and the other received high lux of the same wavelength for five hours exposure).
Fourier transform infrared and ultraviolet spectroscopy were used to detect changes in proteins and lipids of different ocular tissues after the exposure to different wavelengths. Total protein, cholesterol, phospholipids, ions, glucose, ascorbic acid concentration and refractive indices were measured to monitor the changes after visible light exposure.