الفهرس 
AcknowledgmentOnly 14 pages are availabe for public view
 |  | from | 128 |  |  |
| | | from | 128 | | |
Abstract
The bacteria that are currently identified as P. shigelloides were originally isolated, in 1947 by Ferguson and Henderson, from a stool specimen of a person in Michigan from whom no clinical manifestation was reported. In 1954, P. shigelloides was first considered as diarrheaogen i c organism as several Plesiomonas strains were isolated from human cases of gastroenteritis and from wild and domestic animals. Gastrointestinal infections with P. shigelloides apparently occur as a result of ingestion of contaminated food or water. Diarrheal diseases represent the main diseases produced shigelloides in man but the organism appears as an opportunistic pathogen mainly seen in debilitated hosts with impaired immune systems or in the neonates. The rare but sever, extraintestinal infections due to P. shigelloides are in the form of meningitis, sepsis, cellulitis, tonsilitis, arthritis, acute cholecystitis, pyonmetra, endophthalimitis- and septicemia. Water, animals and food are vehicles for the transmission of Plesiomonas to humans. P. shigelloides, until recently, has received little attention from the over medical community all including Egypt when several new sporadic reports its association with disease in man. The performed investigation present was the world indicated for pet animals (dogs & cats), fish & shellfish and shigelloides in humans, in determina t ion of distribution of ~ Alexandria city. water [1] 700 samples were collected from human: A total of 1020 samples were included in this study: 1) 250 rectal swabs from diarrheic patients. 2) 150 stool samples from each of : non-diarrheal children, persons, school handlers. [2] Rectal swabs from 50 dogs & 50 cats. and food [3] One hundred and twenty fish and shellfish of many kinds. [4] One hundred of surface water samples were collected from different bathing beaches and fresh water ways of city. Alexandria Identification the -All the samples were cultured on inositole brilliant green isolated bile of all salts agar. Plesiomonas by testing the non-naemolytic colonies from blood agar for oxidase production. Then the decarboxylation of Ornithin Kaper’s media and more confirmation in the performed using the APi 20 E system. Antibiotic sensitivity test using the single were diffusion disk were performed on all the Plesiomonas strains. Sixty four P. shigelloides were recovered from all the samples. All of them had the typical colonial morphology on the IBBA and gave the typical reaction in the Kaper’ s medium and 5 6 (87.5) E gave the profile 7144204 on the 20 APi system meaning that these isolates were ONPC posi ti ve while 8 (12.5) were ONPG negative. Plesiomonas ( 3 . 2 ) diarrheal recovered from 8 was patients and 4 (2.27) food handlers.