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العنوان
BIOTECHNOLOGICAL STUDIES ON DATE PALM VIA TISSUE CULTURE TECHNIQUES\
الناشر
Cairo University. Faculty of Agriculture. Department of Pomology,
المؤلف
MADBOLY, ESSAM ABDEL-RAHMAN
هيئة الاعداد
مشرف / EISSA,MOHAMED AHMED
مشرف / GOMMAA , AMINA HAMED
مشرف / MADBOLY, ESSAM ABDEL-RAHMAN
مشرف / ESSAM ABDEL-RAHMAN MADBOLY.
تاريخ النشر
2007 .
عدد الصفحات
243p.
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم النبات
تاريخ الإجازة
1/1/2007
مكان الإجازة
جامعة القاهرة - Pomology
الفهرس
Only 14 pages are availabe for public view

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Abstract

This study was carried out at the period of 2002-2006 in the Laboratory of the Central Laboratory for Date Palm Researches and Development (CLDRD), Agriculture Research Center (ARC),Giza, Egypt.
The major objectives of the present study were to in vitro micropropagation protocol for root explants of date palm cv. Sewi and identification of somaclonal variation of date palm through RAPD-PCR technique comparing within plantlets produced from root explants in vitro micropropagation and mother plant. and explants type on in vitro micropropagation of date palm cvs. Sewi, Zaghloul and Samani and identification of somaclonal variation of date palm through RAPD-PCR technique by compared with effect of subculture number of plantlets produced from in vitro micropropagation and mother plant.
This study included three experiments :
1 - Experiment (1): - In vitro micropropagation protocol for root explants of date palm cv. Sewi.
2 - Experiment (2): - Effect of subculture number and explant type on in vitro micropropagation of date palm cvs. Sewi, Zaghloul and Samani
3 - Experiment (3): - Identification of somaclonal variation of date palm through RAPD-PCR technique
1 - Experiment (1): - In vitro micropropagation protocol for root explants of date palm cv. Sewi.
a- Effect of surface sterilization treatments, culture medium, and explant types on survival percentages of date palm cv. Sewi. :-
In this experiment healthy offshoots of Sewi date palm cultivar (semi-dry cultivar) were selected from mother trees.