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العنوان
Comparative studies on antibacterial potential of some plants against pathogenic bacteria /
المؤلف
EL-Namla, Amany Kamal EL-Desouky.
هيئة الاعداد
باحث / أمانى كمال الدسوقى النملة
مشرف / عايدة حافظ عفيفى
مشرف / فتحى إسماعيل على حوقه
مشرف / محمد منصور قاسم
مناقش / أحلام على مصطفى محيسن
مناقش / إيمان حسين صاشور
الموضوع
Phytopathogenic bacteria. Antibacterial activity. Plant oils. Antibiotics. Patogenic bacteria.
تاريخ النشر
2016.
عدد الصفحات
171 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
الزراعية والعلوم البيولوجية (المتنوعة)
تاريخ الإجازة
01/05/2016
مكان الإجازة
جامعة المنصورة - كلية الزراعة - Department of Microbiology
الفهرس
Only 14 pages are availabe for public view

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from 186

Abstract

Objectives : Study of some different plant extracts , also some plant oils and antibiotics on growth and inhibition of pathogenic bacteria and investigate phytochemical analysis for the plant extracts Method and results : Eight various plant were extracts include ( rosemary, orange, camphor, guava, chamomile, onion, garlic and black cumin ) by using 4 solvents ( water-methanol-ethanol-acetone ) to determine antibacterial activityof them against 6 pathogenic bacteria ( E. coli, Salmonella sp., Shigella flexneri, Enterobacter sakazakii, S. aureus and B. cereus ) to determine inhibition zone by using the well diffusion method and determine minimum inhibitory concentration ( MIC ) and minimum bactericidal concentration ( MBC ) by using the serial dilution method. Obtained data showed that different plant extracts of rosemary, orange, camphor and giava were highest effect against all bacteria while chamomile showed moderate effect, onion and garlic showed lower effect. Black cumin did not effect on all bacteria. MIC and MBC for rosemary, the aqueous extract were more than 25 mg / ml for all bacteria while the methanol extract exhibited MIC ( 6.25 mg / ml ) and MBC ( 12.5 mg / ml ) for E. coli, S.sp., S. aureus and B. cereus. Also MIC was( 12.5 mg / ml ) and MBC was ( 25 mg / ml ) for E. sakazakii and S. flexneri . The ethanol extract showed MIC ( 6.25 mg / ml ) and MBC ( 12.5 mg / ml ) for E. coli and S. aureus. MIC was ( 25 mg / ml ) and MBC was ( 25 mg / ml ) for E. sakazakii while Salmonella. sp. was resistance. The acetone extract showed MIC at ( 6.25 mg / ml ) and MBC at ( 12.5 mg / ml ) for S. sp. and E. sakazakii. While MIC was ( 12.5 mg / ml ) and MBC was ( 25 mg / ml ) for E. coli, S. aureus and S. flexneri but MIC was ( 25 mg / ml ) and MBC was ( 25 mg / ml ) for B. cereus . The different plant extracts of orange, camphor, guava, onion and garlic showed MIC and MBC ( 25 mg / ml ).Also studied effect of some plant oils of rosemary, camphor, spearmint, orange, castorbeans, garlic, lemongrass and fenugreek on pathogenic bacteria by using the well diffusion method. The results showed that rosemary oil was best effect followed by orange, camphor, spearmint showed little effect on some bacteria but other plant oils did not show any effect on all bacteria . Five antibiotics were tested against pathogenic bacteria by using the well diffusion method. The results showed that all bacteria were sensitive to antibiotics tetracycline and chloramphenicol except Salmonella. sp. was resistance to them. S. aureus and E. sakazakii were sensitive to ampicillin while E. coli was moderate but B. cereus, S. sp., S. flexneri were resistance.E. coli, S. flexneri, E. sakazakii and S. aureus were sensitive to cephalexin while B. cereus was moderate. Salmonella. sp. was resistance. The phytochemical analysis was carried by using qualitative analysis. The results showed presence active compound such as terpens, alkaloids, flavonoids, tannins, saponins and glycosides . Conclusion : The plant extracts oe rosemary, orange, camphor and guava showed the highest antibacterial activity. The plant oils of rosemary, camphor, spearmint and orange showed weak effect while other plant oils did not show any effect. E. sakazakii was high sensitivity to amofluxin, S. aureus was moderate while other bacteria were resistance. S. sp. was resistance to all antibiotics .