الفهرس 
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Abstract
This study was planned to realize the possibility of using natural antioxidant agents (Black seed oil &Olive oil mixture) in improving the adverse effect of polluted water (LMW) on fish and for protection of fish from oxidative stress. So our study tends to investigate water quality in both lake water and tap water, evaluate some hematological, biochemical, oxidative , antioxidant and immunological parameters in tilapia reared in different water quality (Lake Manzala Water (LMW), Tap Water), some histopathological studies on liver and gills as well as bacterial challange with A. hydrophila after the experimental period Analysis of Lake Manzala water and tap water reflected that Lake water undergoes several types of pollutant. Whilst some clinicopathological studies on fish within the experimental period clarified that LMW has a negative effect on fish that appeared as hypocytic hypochromic anemia, decrease liver function, alternation in immune, oxidative and antioxidant markers. Whereas oil treatment ameliorate the effect of polluted water on fish, Furthermore, histopathological examination of liver and gills releaved pathological changes. On the other hand oil treated fish in LMW reflected the ameliorative effect of BSO and OO. Also oil treated fish in tap water showed no pathological lesion in both organs. Also, mortality rate decreased in fish treated with oil in lake water and challanged with A. hydrophila in compare with the mortality rate in untreated fish in LW. Moreover the mortality rate in treated fish in tap water displayed the lowest percent.