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Enterobacter cloacaeOnly 14 pages are availabe for public view
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Abstract
Nosocomial infection due to Enterobacter species are increasing in frequency especially in ICUs, with increasing resistant to antibiotics causing significant morbidity and mortality. This resistance is mainly due to production of β-lactamase enzymes leading to failure of therapy by β-lactam drugs. Aim of the work:To isolate E. cloacae strains from nosocomially infected patients in ICUs of MUHs, detect MBL-producing strains, determine bla VIM-1- harbouring isolates, and determine risk factors for infection by VIM-1-MBL-producing strains.Research plan: A total of 1500 samples were collected from patients fulfilling the criteria of nosocomial infection. Isolation and identification of E. cloacae strains was done by conventional culture methods, Gram staining, and biochemical reactions, and confirmed by API 20 E test. Antibiotic sensitivity test was performed to all isolated E. cloacae strains to detect imipenem-resistant isolates. Imipenem-resistant isolates were selected for further testing including: Phenotypic detection of MBL production by imipenem-EDTA double disc synergy test. PCR for detection of bla VIM-1 gene in MBL-producing strains. Results:E. cloacae isolates accounted for 3.4 % of all isolated pathogens. Imipenem-resistant E.cloacae isolates were 33.3% of all isolated E. cloacae strains. MBLs production which was detected in 27.5 % of E. cloacae isolates and bla VIM-1 gene was detected in 7.8 % of E. cloacae isolates. Conclusion:The prevalence VIM-MBL-producing E. cloacae isolates causing infections in this study is low with increase in antibiotic resistance in ICUs with increase in mortality rates.