الفهرس 
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Abstract
The aim of this work is to carry out phytochemical and biological study of secondary metabolite from the leaves and bark of Salix babylonicabelonging to family salicaceaein order to discover new compounds with biological activities. In this study, eleven compounds were isolated and identified using various spectroscopic techniques.Also, the isolated compounds were tested as antioxidant activities, antimicrobial activities, antiquorum activities and cytotoxic activities against Hepg-2 and MCF- 7 carcinoma cell lines. Part I:Phytochemical study of the leaves and bark of Salix babylonica The air-dried and powdered leaves and bark of Salix babylonica were extracted by maceration with methanol then concentrated to a syrupy consistency which extracted successively with petroleum ether, methylene chloride, ethyl acetate and butanol.Isolation carried outusing column chromatography on normal phase silica using different organic solvents with increasing polarity. Then, the isolated compounds were identified using various spectroscopic techniques including; UV, 1D- and 2D-NMR, ESIMS and HRESIMS. as (salicin, populoside B and acmophylline B),(methyl trans-4-hydroxycinnamate and 1-p-coumaroyl- β- D- glucopyranoside), (catechol and catechin), (luteolin, apigenin 7- O-β-D-(4”-p-coumaroyl) glucoside and luteolin 7- O-β-D- glucoside) and one new flavonone (chryseoriol 7- O-β-D-glucuronide methyl ester) is reported in the present study for the first time from a natural source. Part II: Biological study of different extracts, fractions and isolated compounds from Salix babylonica I- Antioxidant activityThe ethyl acetate fraction of the methanolic extract of the bark possessed the highest phenolic also the highest antioxidant activity amongst all the tested fractions, this may be attributed to catechin. II- Antimicrobial activity:luteolin has the highest antibacterial activity against E. coliamongst all tested compounds. Methyl trans-4-hydroxycinnamate showed the strongest antibacterial activityagainst K. pneumoniaamongst all tested compounds. Populoside B and 1-p-coumaroyl-β-D- glucopyranoside showed the strongest antibacterial activityagainst P. aeruginosa amongst all tested compounds.Chryseoriol 7-O-β-D-glucuronide methyl ester, luteolin 7-O-β-D- glucoside, populoside B and 1-p-coumaroyl-β-D- glucopyranoside showed the strongestantibacterial activityagainst S. aureus. luteolin 7-O-β-D- glucoside, methyl trans-4-hydroxycinnamate, catechin and populoside B showed moderate antifungal activity against C. albicans. III- Anti-quorum sensing activity: Catechin, luteolin, chryseoriol 7-O-β-D- glucuronide methyl ester, luteolin 7-O-β-D- glucoside, methyl trans-4-hydroxycinamat and 1-p-coumaroyl-β-D- glucopyranoside showed high antiquarum sensing activity against Ch. Violaceum.