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العنوان
Cytogenetical and molecular studies on genus sesbania scop. (Fabaceae) /
المؤلف
Said-Ahmed, Eman Sedky Othman.
هيئة الاعداد
باحث / مان صدقى عثمان سيد أحمد
مشرف / ماجدة إبراهيم سليمان
مشرف / لندا زخاري سمعان
مناقش / ماجدة إبراهيم سليمان
الموضوع
Plant cytology. Botany. Genetics.
تاريخ النشر
2019.
عدد الصفحات
171 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم النبات
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة المنصورة - كلية العلوم - النبات
الفهرس
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Abstract

The aim of this study is to demonstrate the genetic diversity within and among two species Sesbania Scop. (Sesbania sesban (L.) Merrill which is perennial taxon and Sesbania sericea (Willd.) Link which is annual taxon) collected from ten accessions growing in natural habitats in Egypt based on different genetic markers as: Soil analysis (physical and chemical), Phytochemical analysis, cytological studies (chromosome count, karyotype measurements and chromosomal abnormalities), Biochemical analysis via seed protein electrophoresis (SDS-PAGE) and Molecular studies via Random Amplified Polymorphic DNA (RAPD marker) and Inter Simple Sequence Repeats (ISSR marker).The physicochemical analysis of soil samples indicate a variation in values according to the different nature of ten accessions sampled.The main results: Soil physical analysis include soil texture and porosity while, chemical analysis includes Hydrogen ion concentration (PH), Electric conductivity (E.C), Soluble anions (chloride, sulphate, carbonate and bicarbonate) and extractable cations (sodium, potassium, calcium and magnesium) and total soluble salts (T.S.S). The physical properties revealed that soil texture of all studied taxa was sandy clay and loamy soil. Soil porosity where the highest value 32.00% in annual Sesbania sericea (Ssa2, Ssa4, Ssa5) and the lowest value 16.12% in Sesbania sesban perennial (Ssp3). The soil reaction PH value exhibited slightly alkaline to highly alkaline. Sesbania sesban (perennial) had the highest values of electrical conductivity (E.C.), chloride, extractable cations and total soluble salt while Sesbania sericea (annual) had the highest values of sulphates and bicarbonates. The loss of the base cations may be due to the higher amounts of leaching because of increased exposure to weather with less dense vegetation, that also result in less basic soil.